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Home Assembling and Scaffolding

Generate Scaffolds by using Paired-End Short Reads

De Novo assembling of NGS short reads tends to generate large number of short contig sequences. As these contigs are not located in the genome, it is difficult to use them. GT provides the "Scaffolding" function that connect such large number of contig sequences into smaller number of scaffold sequences. GT also provides a further function to connect all the scaffolds into one big sequence.

Data to be used.

Contig sequences in multiple FastA format.

Paired-End short reads sequences in (Solexa, SOLiD, 454 format) files.

Software to be used.


The scaffold viewer of GenomeTraveler

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